Recognising, preventing and alleviating pain and distress in laboratory animals is a key responsibility of all those involved in their care and use. This workshop is one of two linked workshops and is focussed on assessing and alleviating post-operative pain and distress in laboratory rodents and rabbits. This workshop (Workshop 2) will take place in June 2025, and features pre-recorded and live webinars, along with e-learning modules. The live sessions aim to provide practical tuition in pain scoring, and application of pain and distress alleviation strategies to a range of different surgical procedures. Revised and updated content will include new recommendations on use of local anaesthetics in rodents, and discuss the problems of dose selection of NSAIDs in mice.